Difference between revisions of "Part:BBa K1789008:Design"
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NJU_CHINA finished the primary construction of this part and edited the vectors provided by the Golden Gate TALEN and TAL Effector Kit 2.0 (adding corresponding cutting sites to be specific) to meet the RFC10 bio-brick standard. | NJU_CHINA finished the primary construction of this part and edited the vectors provided by the Golden Gate TALEN and TAL Effector Kit 2.0 (adding corresponding cutting sites to be specific) to meet the RFC10 bio-brick standard. | ||
| − | [[File:TALE1.jpg]] | + | [[File:TALE1.jpg|200px|]] |
Fig. 1 The Sequence of TALE1 cDNA and deduced amino acids | Fig. 1 The Sequence of TALE1 cDNA and deduced amino acids | ||
The protocol can be reached in our wiki | The protocol can be reached in our wiki | ||
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===Source=== | ===Source=== | ||
Revision as of 07:48, 18 September 2015
TALE1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2082
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1075
Design Notes
This part is designed based on the sequence of BM1 in our project.
NJU_CHINA finished the primary construction of this part and edited the vectors provided by the Golden Gate TALEN and TAL Effector Kit 2.0 (adding corresponding cutting sites to be specific) to meet the RFC10 bio-brick standard.
Fig. 1 The Sequence of TALE1 cDNA and deduced amino acids
The protocol can be reached in our wiki
Source
This part is built by NJU_CHINA using GATEWAY technique. We standardized this part by ourselves.