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Status: 500 Content-type: text/html

Software error:

Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84.
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84.
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 85.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12.
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Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9.
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Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
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Usage and Biology

Curdlan is a bacterial polysaccharide (linear (1->3)-β-glucan) that has been of significant recent interest due to its valuable properties and its bioactivity. In fact, Curdlan belongs to the class of biological response modifiers that enhance or restore normal immune defenses. For example, it can have antitumor, anti-infective, anti-inflammatory, and anticoagulant activities (see other properties of Curdlan). In particular, this (1->3)-β-glucan can stimulate plants' immune system.
Our project is focused on the production of curdlan for stimulate plant defense system against Downy Mildew.

The molecular genetics of curdlan production have been investigated in Agrobacterium sp. ATCC31749. Studies identified three genes (crdA, crdS and crdC) that are essential for curdlan production The crdASC genes occupy a contiguous 4,948 bp region of the genome ( the crdS gene is flanked by the crdA and crdC genes).

CrdC assists translocation of the nascent polymer across across the periplasm.

Note : This protein has not really well described in literature.

For our project, we wanted to produce curdlan by E.coli, so this part has been made with a codon optimization of the natural crdC sequence for E.coli.