Difference between revisions of "Part:BBa K1769005"
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MtrB, an outer membrane protein from Shewanella oneidensis MR-1, is required for proper incorporation of cytochromes into the outer membrane. This protein is important in stablizing OcmA and OcmB. two outer membrane protein that plays an essential role in netal reduction pathway in S.oneidensis and is also important in genetraing current in microbialfuel cell. We reintroduce this gene into a mtrB gene knockout mutant Shewanella JG700 to create a sensor with electric current as reporter. With a suitable inducible promoter, mtrB will be activated whenever the promoter is induced. | MtrB, an outer membrane protein from Shewanella oneidensis MR-1, is required for proper incorporation of cytochromes into the outer membrane. This protein is important in stablizing OcmA and OcmB. two outer membrane protein that plays an essential role in netal reduction pathway in S.oneidensis and is also important in genetraing current in microbialfuel cell. We reintroduce this gene into a mtrB gene knockout mutant Shewanella JG700 to create a sensor with electric current as reporter. With a suitable inducible promoter, mtrB will be activated whenever the promoter is induced. | ||
| + | <img src="https://static.igem.org/mediawiki/parts/9/95/Elecmtrb.jpg" style="align:left; width:40%; padding-bottom:2%"> | ||
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Revision as of 07:34, 18 September 2015
MtrB Outer Membrane Structural Protein
MtrB, an outer membrane protein from Shewanella oneidensis MR-1, is required for proper incorporation of cytochromes into the outer membrane. This protein is important in stablizing OcmA and OcmB. two outer membrane protein that plays an essential role in netal reduction pathway in S.oneidensis and is also important in genetraing current in microbialfuel cell. We reintroduce this gene into a mtrB gene knockout mutant Shewanella JG700 to create a sensor with electric current as reporter. With a suitable inducible promoter, mtrB will be activated whenever the promoter is induced.
<img src="
" style="align:left; width:40%; padding-bottom:2%">
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]