Difference between revisions of "Part:BBa K1640008:Design"

Revision as of 07:26, 18 September 2015

psbMZH

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Genes required for photosystem II were selected using biocyc entry on Chlamydomonas reinhardtii photosystem II complex. Sequences were obtained from NCBI, targeting sequences were identified in nuclear using ChloroP, and removed, with an ATG added. Resulting sequences were codon optimized for e. coli expression using Optimizer, avoiding EcoRI, XbaI, SpeI and XbaI sites.

Ribosome binding sites were added in front of each CDS, and a promoter was added to biobricks which form the first part of their intended operon.

References

Bentley, F. K., Luo, H., Dilbeck, P., Burnap, R. L., & Eaton-Rye, J. J. (2008). Effects of Inactivating psbM and psbT on Photodamage and Assembly of Photosystem II in Synechocystis sp. PCC 6803†. Biochemistry, 47(44), 11637-11646.

Ferreira, K. N., Iverson, T. M., Maghlaoui, K., Barber, J., & Iwata, S. (2004). Architecture of the Photosynthetic Oxygen-Evolving Center. Science, 303(5665), 1831-1838. doi:10.1126/science.1093087

Komenda, J., Lupínková, L., & Kopecký, J. (2002). Absence of the psbH gene product destabilizes photosystem II complex and bicarbonate binding on its acceptor side in Synechocystis PCC 6803. European Journal of Biochemistry, 269(2), 610-619. doi:10.1046/j.0014-2956.2001.02693.x

Mayes, S. R., Dubbs, J. M., Vass, I., Hideg, E., Nagy, L., & Barber, J. (1993). Further characterization of the psbH locus of Synechocystis sp. PCC 6803: inactivation of psbH impairs QA to QB electron transport in photosystem 2. Biochemistry, 32(6), 1454-1465.

Minagawa, J., & Takahashi, Y. (2004). Structure, function and assembly of Photosystem II and its light-harvesting proteins. Photosynthesis Research, 82(3), 241-263. doi:10.1007/s11120-004-2079-2

Summer, E. J., Schmid, V. H., Bruns, B. U., & Schmidt, G. W. (1997). Requirement for the H phosphoprotein in photosystem II of Chlamydomonas reinhardtii. Plant physiology, 113(4), 1359-1368.

Swiatek, M., Kuras, R., Sokolenko, A., Higgs, D., Olive, J., Cinque, G., . . . Bassi, R. (2001). The chloroplast gene ycf9 encodes a photosystem II (PSII) core subunit, PsbZ, that participates in PSII supramolecular architecture. The Plant Cell, 13(6), 1347-1368.

Umate, P., Schwenkert, S., Karbat, I., Dal Bosco, C., Mlcòchová, L., Volz, S., . . . Meurer, J. (2007). Deletion of PsbM in tobacco alters the QB site properties and the electron flow within photosystem II. Journal of Biological Chemistry, 282(13), 9758-9767.

Vener, A. V., Harms, A., Sussman, M. R., & Vierstra, R. D. (2001). Mass spectrometric resolution of reversible protein phosphorylation in photosynthetic membranes ofArabidopsis thaliana. Journal of Biological Chemistry, 276(10), 6959-6966.

@@ Line 9: / Line 9: @@
 Genes required for photosystem II were selected using <html><a href=http://www.biocyc.org/CHLAMY/NEW-IMAGE?type=ENZYME&object=CPLX4LZ-235>biocyc entry on <i>Chlamydomonas reinhardtii</i> photosystem II complex</a>. Sequences were obtained from NCBI, targeting sequences were identified in nuclear using <a href=http://www.cbs.dtu.dk/services/ChloroP>ChloroP</a>, and removed, with an ATG added. Resulting sequences were codon optimized for e. coli expression using <a href=http://genomes.urv.es/OPTIMIZER/>Optimizer</a></html>, avoiding EcoRI, XbaI, SpeI and XbaI sites.
+Ribosome binding sites were added in front of each CDS, and a promoter was added to biobricks which form the first part of their intended operon.
 ===References===