Difference between revisions of "Part:BBa K1813012"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1813012 short</partinfo>
 
<partinfo>BBa_K1813012 short</partinfo>
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A construct used to achieve the overexpression of CYP6G1(( <html><b><a href="https://parts.igem.org/Part:BBa_K1813004">BBa_K181304</b></a></html>)  
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A construct used to achieve the overexpression of CYP6G1(<html><b><a href="https://parts.igem.org/Part:BBa_K1813004">BBa_K181304</b></a></html>) <br>
 
Structure: ptac:: CYP6G1:: terminator.
 
Structure: ptac:: CYP6G1:: terminator.
  

Revision as of 06:33, 18 September 2015

CYP6G1 Expression Cassette A construct used to achieve the overexpression of CYP6G1(BBa_K181304)
Structure: ptac:: CYP6G1:: terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1021
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1030


Background

Powered by the constitutive bacterial promoter ptac, this construct allows the indiscriminate production of CYP6G1 by a bacterium harbouring this construct. This construct was assembled by Michael VanInsberghe, and is codon optimized for E. coli .
It is used in BBa_K1813025 and BBa_K1813033.