Difference between revisions of "Part:BBa K1813022"

 
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<partinfo>BBa_K1813022 short</partinfo>
 
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<partinfo>BBa_K1813022 parameters</partinfo>
 
<partinfo>BBa_K1813022 parameters</partinfo>
 
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<h2>Background of <i>Ptac NicE Term</i></h2>
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<h4>Part Description:</h4>
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<p>
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</p>
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<h4>Part Origin</h4>
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<p>where part originated and <i>Genus species</i> more words if needed</p>
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<h4>Part Function</h4>
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<p>part functionstuff </p>
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<h4>Design and Acquisition</h4>
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<p>
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After synthesizing a codon-optimized NicE, we used standard assembly to created a composite part composed of NicE driven by the Ptac promoter (BBa_K1813037) and flanked by a double terminator (BBa_B0014). The tac promoter contains a lac operator sequence that can be bound by LacI, the lac repressor protein, allowing inducible expression by Isopropyl β-D-1-thiogalactopyranoside (IPTG). Our NicE expression cassette was assembled behind a lacI cassette (BBa_K1813019) to give us the ability to control the expression of NicE. All nicE constructs are contained within standard pSB1C3 vectors.</p>
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<h4>Experience</h4>
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<p><p>

Revision as of 05:32, 18 September 2015

nicE Expression Cassette with LacI Reversed

LacI Reversed nicE


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2054
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1469


Background of Ptac NicE Term

Part Description:


Part Origin

where part originated and Genus species more words if needed

Part Function

part functionstuff

Design and Acquisition

After synthesizing a codon-optimized NicE, we used standard assembly to created a composite part composed of NicE driven by the Ptac promoter (BBa_K1813037) and flanked by a double terminator (BBa_B0014). The tac promoter contains a lac operator sequence that can be bound by LacI, the lac repressor protein, allowing inducible expression by Isopropyl β-D-1-thiogalactopyranoside (IPTG). Our NicE expression cassette was assembled behind a lacI cassette (BBa_K1813019) to give us the ability to control the expression of NicE. All nicE constructs are contained within standard pSB1C3 vectors.

Experience

<p>