Difference between revisions of "Part:BBa K1813021"

 
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<partinfo>BBa_K1813021 short</partinfo>
 
<partinfo>BBa_K1813021 short</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K1813021 parameters</partinfo>
 
<partinfo>BBa_K1813021 parameters</partinfo>
 
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<h2>Background of <i>LacI Ptac NicD Term</i></h2>
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<h4>Part Description:</h4>
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<p>
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</p>
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<h4>Part Origin</h4>
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<p>where part originated and <i>Genus species</i> more words if needed</p>
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<h4>Part Function</h4>
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<p>part functionstuff </p>
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<h4>Design and Acquisition</h4>
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<p>
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After synthesizing a codon-optimized NicD, we used standard assembly to created a composite part composed of NicD driven by the Ptac promoter (BBa_K1813037) and flanked by a double terminator (BBa_B0014). The tac promoter contains a lac operator sequence that can be bound by LacI, the lac repressor protein, allowing inducible expression by Isopropyl β-D-1-thiogalactopyranoside (IPTG). Our NicD expression cassette was assembled behind a lacI cassette (BBa_K1813019) to give us the ability to control the expression of NicD. All nicD constructs are contained within standard pSB1C3 vectors.</p>
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<h4>Experience</h4>
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<p><p>

Revision as of 05:31, 18 September 2015

nicD Expression Cassette with LacI Reversed

LacI Reversed nicD


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2141
  • 1000
    COMPATIBLE WITH RFC[1000]


Background of LacI Ptac NicD Term

Part Description:


Part Origin

where part originated and Genus species more words if needed

Part Function

part functionstuff

Design and Acquisition

After synthesizing a codon-optimized NicD, we used standard assembly to created a composite part composed of NicD driven by the Ptac promoter (BBa_K1813037) and flanked by a double terminator (BBa_B0014). The tac promoter contains a lac operator sequence that can be bound by LacI, the lac repressor protein, allowing inducible expression by Isopropyl β-D-1-thiogalactopyranoside (IPTG). Our NicD expression cassette was assembled behind a lacI cassette (BBa_K1813019) to give us the ability to control the expression of NicD. All nicD constructs are contained within standard pSB1C3 vectors.

Experience

<p>