Difference between revisions of "Part:BBa K1833008"

 
 
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<partinfo>BBa_K1833008 short</partinfo>
 
<partinfo>BBa_K1833008 short</partinfo>
  
This part produces a synthetic repressor in response to T7 RNA Polymerase. This synthetic repressor consists of a fusion of two DNA binding domains connected with a linker that is sensitive to matrix metalloprotease 9, and it represses the promoter described by parts BBa_K1833001 and BBa_K1833003. For more information on this part, visit 2015.igem.org/Team:Pitt/Protease/Project.
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This part produces a synthetic repressor in response to T7 RNA Polymerase. This synthetic repressor consists of a fusion of two DNA binding domains connected with a linker that is sensitive to matrix metalloprotease 9, and it represses the promoter described by parts BBa_K1833001 and BBa_K1833003. For more information on the use of this part, visit <html><a href="http://2015.igem.org/Team:Pitt/Protease/Project">the 2015 Pitt iGEM page.</a></html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 05:29, 18 September 2015

pT7-MMP9 sensitive repressor

This part produces a synthetic repressor in response to T7 RNA Polymerase. This synthetic repressor consists of a fusion of two DNA binding domains connected with a linker that is sensitive to matrix metalloprotease 9, and it represses the promoter described by parts BBa_K1833001 and BBa_K1833003. For more information on the use of this part, visit the 2015 Pitt iGEM page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 64
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 297
    Illegal NgoMIV site found at 696
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 194
    Illegal SapI.rc site found at 53