Difference between revisions of "Part:BBa K1795001"
| Line 3: | Line 3: | ||
This sequence codes for dCas9 and is codon optimized for E. Coli. It is under the promoter R0010 and has RBS B0034 and DT B0015. | This sequence codes for dCas9 and is codon optimized for E. Coli. It is under the promoter R0010 and has RBS B0034 and DT B0015. | ||
| − | [[Image:WMIntegratorCassetteMap.png]] | + | [[Image:WMIntegratorCassetteMap.png|thumb]] |
When co-transformed with RFP and BBa_K1795002 97% reduction of RFP was observed. | When co-transformed with RFP and BBa_K1795002 97% reduction of RFP was observed. | ||
Revision as of 05:26, 18 September 2015
E. Coli Codon Optimized fdCAS9 under R0010
This sequence codes for dCas9 and is codon optimized for E. Coli. It is under the promoter R0010 and has RBS B0034 and DT B0015.
When co-transformed with RFP and BBa_K1795002 97% reduction of RFP was observed.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1016
Illegal PstI site found at 2642
Illegal PstI site found at 3884 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1016
Illegal PstI site found at 2642
Illegal PstI site found at 3884 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1216
Illegal BglII site found at 1272
Illegal BglII site found at 1414
Illegal BglII site found at 1962
Illegal BglII site found at 3802
Illegal BglII site found at 4041
Illegal BamHI site found at 3604
Illegal BamHI site found at 3965 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1016
Illegal PstI site found at 2642
Illegal PstI site found at 3884 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1016
Illegal PstI site found at 2642
Illegal PstI site found at 3884
Illegal NgoMIV site found at 4186
Illegal AgeI site found at 3530 - 1000COMPATIBLE WITH RFC[1000]