Difference between revisions of "Part:BBa K1833009"
(Created page with "__NOTOC__ <partinfo>BBa_K1833009 short</partinfo> This part generates the DNA binding domain of the phage 434 cI repressor, mutated to have the specificity of the phage P22 repr...") |
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| − | This part generates the DNA binding domain of the phage 434 cI repressor, mutated to have the specificity of the phage P22 repressor. Without the C-terminus, it cannot dimerize and form the fully functional repressor. This part was used as a negative control for repression of a synthetic promoter (BBa_K1833001). | + | This part generates the DNA binding domain of the phage 434 cI repressor, mutated to have the specificity of the phage P22 repressor. Without the C-terminus, it cannot dimerize and form the fully functional repressor. This part was used as a negative control for repression of a synthetic promoter (BBa_K1833001). For more information on the use of this part, visit <html><a href="http://2015.igem.org/Team:Pitt/Protease/Project">the 2015 Pitt iGEM page.</a></html> |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 05:25, 18 September 2015
T7 promoter -> 434 cI N terminus with P22 repressor specificity
This part generates the DNA binding domain of the phage 434 cI repressor, mutated to have the specificity of the phage P22 repressor. Without the C-terminus, it cannot dimerize and form the fully functional repressor. This part was used as a negative control for repression of a synthetic promoter (BBa_K1833001). For more information on the use of this part, visit the 2015 Pitt iGEM page.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]