Difference between revisions of "Part:BBa K1833005"

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<partinfo>BBa_K1833005 short</partinfo>
 
<partinfo>BBa_K1833005 short</partinfo>
  
This part generates the DNA binding domain of the phage 434 repressor. Without the C-terminus, it cannot dimerize and form the fully functional repressor. This part was used as a negative control for repression of a synthetic promoter (BBa_K1833001). For more information on the use of this part, visit [http://2015.igem.org/Team:Pitt/Protease/Project].
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This part generates the DNA binding domain of the phage 434 repressor. Without the C-terminus, it cannot dimerize and form the fully functional repressor. This part was used as a negative control for repression of a synthetic promoter (BBa_K1833001). For more information on the use of this part, visit <nowiki><a href="http://2015.igem.org/Team:Pitt/Protease/Project">the 2015 Pitt iGEM page.</a></nowiki>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 05:24, 18 September 2015

pT7-Phage 434 repressor N-terminus

This part generates the DNA binding domain of the phage 434 repressor. Without the C-terminus, it cannot dimerize and form the fully functional repressor. This part was used as a negative control for repression of a synthetic promoter (BBa_K1833001). For more information on the use of this part, visit <a href="http://2015.igem.org/Team:Pitt/Protease/Project">the 2015 Pitt iGEM page.</a>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 64
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]