Difference between revisions of "Part:BBa K1859020"
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Therefore we designed some linkers to fix the folding of the protein.  
 
Therefore we designed some linkers to fix the folding of the protein.  
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Then, we combined circular parts, the 3'side of the intron[BBa_    ] and the 5'side of the intron[BBa_], with those linkers and made them parts.
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Then, we combined circular parts, the 3'side of the intron
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332005" > [BBa_K1332005] </a>
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and the 5'side of the intron
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<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332003" > [BBa_K1332003] </a>
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, with those linkers and made them parts.
  
  

Revision as of 04:44, 18 September 2015

Histidine tag and RFP linker [GGSGGS*2] semi-permanent generator

We designed this generator[K1859020] to express long-chain protein with keeping functionality of the protein.

In the study of iGEM Gifu 2014, we made long-chain protein from Circular mRNA, but it lost functionality. It is thought that folding of the protein failed for a cause．

Therefore we designed some linkers to fix the folding of the protein. Then, we combined circular parts, the 3'side of the intron [BBa_K1332005] and the 5'side of the intron [BBa_K1332003] , with those linkers and made them parts. The generator consists of リンカーを結合させたサーキュラーパーツ([BBa_]と[BBa_])、His-RFP without stop codon[BBa_K1332002]、Lacl[]、RBS[]、DT[].

しかし、このgeneratorを導入した大腸菌で発現させたRFPは、蛍光を発するどころか、タンパク質の長鎖化さえされなかった。