Difference between revisions of "Part:BBa K1859020"
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In the study of iGEM Gifu 2014, we made long-chain protein from Circular mRNA, but it lost functionality.  
 
In the study of iGEM Gifu 2014, we made long-chain protein from Circular mRNA, but it lost functionality.  
 
It is thought that folding of the protein failed for a cause．  
 
It is thought that folding of the protein failed for a cause．  
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Therefore we designed some linkers to fix the folding of the protein.  
 
Therefore we designed some linkers to fix the folding of the protein.  
 
Then, we combined circular parts, the 3'side of the intron[BBa_    ] and the 5'side of the intron[BBa_], with those linkers and made them parts.
 
Then, we combined circular parts, the 3'side of the intron[BBa_    ] and the 5'side of the intron[BBa_], with those linkers and made them parts.
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The generator consists of リンカーを結合させたサーキュラーパーツ([BBa_]と[BBa_])、His-RFP without stop codon[BBa_K1332002]、Lacl[]、RBS[]、DT[].
 
The generator consists of リンカーを結合させたサーキュラーパーツ([BBa_]と[BBa_])、His-RFP without stop codon[BBa_K1332002]、Lacl[]、RBS[]、DT[].
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しかし、このgeneratorを導入した大腸菌で発現させたRFPは長鎖化されない。
 
しかし、このgeneratorを導入した大腸菌で発現させたRFPは長鎖化されない。
 
</p>
 
</p>

Revision as of 04:38, 18 September 2015

Histidine tag and RFP linker [GGSGGS*2] semi-permanent generator

We designed this generator[K1859020] to express long-chain protein with keeping functionality of the protein.

In the study of iGEM Gifu 2014, we made long-chain protein from Circular mRNA, but it lost functionality. It is thought that folding of the protein failed for a cause．

Therefore we designed some linkers to fix the folding of the protein. Then, we combined circular parts, the 3'side of the intron[BBa_ ] and the 5'side of the intron[BBa_], with those linkers and made them parts. The generator consists of リンカーを結合させたサーキュラーパーツ([BBa_]と[BBa_])、His-RFP without stop codon[BBa_K1332002]、Lacl[]、RBS[]、DT[].

しかし、このgeneratorを導入した大腸菌で発現させたRFPは長鎖化されない。