Difference between revisions of "Part:BBa K1859002:Experience"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 19: | Line 18: | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
<!-- DON'T DELETE --><partinfo>BBa_K1859002 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K1859002 EndReviews</partinfo> | ||
| + | |||
| + | <html> | ||
| + | <p> | ||
| + | <a href= "http://2015.igem.org/Team:Gifu" > [iGEM gifu 2015] | ||
| + | </a> . | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | We attached this part[BBa_1859002] to 5 'sides of | ||
| + | His-RFP without stop codon | ||
| + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332002" > [BBa_K1332002] | ||
| + | </a> . | ||
| + | |||
| + | Then, it was connected to 5 'sides of | ||
| + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859005" > [BBa_K1859005] | ||
| + | </a> | ||
| + | . | ||
| + | |||
| + | In addition,Lacl | ||
| + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a> | ||
| + | , RBS | ||
| + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0034" >[BBa_B0034] </a> | ||
| + | and DT | ||
| + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_B0015] </a> | ||
| + | was conbined with it, making | ||
| + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859028" >[BBa_K1859028] </a> | ||
| + | . | ||
| + | |||
| + | When this device was expressed in E.coli, Curcular mRNA including the linker was made. | ||
| + | The efficiency that mRNA is circularized changed by sequence of the mRNA. | ||
| + | We let mRNA of RFP circularize and produced a long-chain protein, but the functionality was lost. | ||
| + | It is a problem to make a long-chain protein with keeping functionality. | ||
| + | </p> | ||
| + | |||
| + | |||
| + | |||
| + | </html> | ||
Revision as of 03:52, 18 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1859002
User Reviews
UNIQf25f6b30cc6e08a5-partinfo-00000000-QINU UNIQf25f6b30cc6e08a5-partinfo-00000001-QINU
We attached this part[BBa_1859002] to 5 'sides of His-RFP without stop codon [BBa_K1332002] . Then, it was connected to 5 'sides of [BBa_K1859005] . In addition,Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] was conbined with it, making [BBa_K1859028] . When this device was expressed in E.coli, Curcular mRNA including the linker was made. The efficiency that mRNA is circularized changed by sequence of the mRNA. We let mRNA of RFP circularize and produced a long-chain protein, but the functionality was lost. It is a problem to make a long-chain protein with keeping functionality.