Difference between revisions of "Part:BBa K1859001:Experience"
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and DT | and DT | ||
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_B0015] </a> | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_B0015] </a> | ||
| − | was conbined with them, making [BBa_K1859027] and [ | + | was conbined with them, making |
| + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859027" >[BBa_K1859027] </a> | ||
| + | and | ||
| + | <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859025" >[BBa_K1859025] </a> | ||
| + | . | ||
When these devices were expressed in E.coli, Curcular mRNA including the linker was made. | When these devices were expressed in E.coli, Curcular mRNA including the linker was made. | ||
Revision as of 03:48, 18 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1859001
User Reviews
UNIQ7045aa664032e4a5-partinfo-00000000-QINU UNIQ7045aa664032e4a5-partinfo-00000001-QINU
We attached this part[BBa_1859001] to 5 'sides of His-RFP without stop codon [BBa_K1332002] . Then, it was connected to each 5 'sides of [BBa_K1859004] and [BBa_K1859006] . In addition,Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] was conbined with them, making [BBa_K1859027] and [BBa_K1859025] . When these devices were expressed in E.coli, Curcular mRNA including the linker was made. The efficiency that mRNA is circularized changed by sequence of the mRNA. We let mRNA of RFP circularize and produced a long-chain protein, but the functionality was lost. It is a problem to make a long-chain protein with keeping functionality.