Difference between revisions of "Part:BBa K1859001:Experience"

Revision as of 03:43, 18 September 2015

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Applications of BBa_K1859001

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UNIQ8341f5320ff430dc-partinfo-00000000-QINU UNIQ8341f5320ff430dc-partinfo-00000001-QINU

[iGEM gifu 2015] .

We attached this part[BBa_1859001] to 5 'sides of His-RFP without stop codon [BBa_K1332002] . Then, it was connected to each 5 'sides of [BBa_K1859004] and [BBa_K1859006] . In addition,Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] was conbined with them, making [BBa_K1859027] and [BBa_1859025]. When these devices were expressed in E.coli, Curcular mRNA including the linker was made. The efficiency that mRNA is circularized changed by sequence of the mRNA. We let mRNA of RFP circularize and produced a long-chain protein, but the functionality was lost. It is a problem to make a long-chain protein with keeping functionality.

@@ Line 38: / Line 38: @@
   </a>.
-In addition,Lacl[],RBS[] and DT[] was conbined with them, making [BBa_K1859025] and [BBa_1859024].
+In addition,Lacl
+<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_R0010] </a>
+, RBS
+<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0034" >[BBa_B0034] </a>
+ and DT
+<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010" >[BBa_B0015] </a>
+ was conbined with them, making [BBa_K1859027] and [BBa_1859025].
-When this device was expressed in E.coli, Curcular mRNA including the linker was made.
+When these devices were expressed in E.coli, Curcular mRNA including the linker was made.
 The efficiency that mRNA is circularized changed by sequence of the mRNA.
 We let mRNA of RFP circularize and produced a long-chain protein, but the functionality was lost.