Difference between revisions of "Part:BBa K1859001:Experience"

Revision as of 03:16, 18 September 2015

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Applications of BBa_K1859001

User Reviews

UNIQ92e58d910a46e753-partinfo-00000000-QINU UNIQ92e58d910a46e753-partinfo-00000001-QINU

[iGEM gifu 2015] .

We attached this part[BBa_1859001] to 5 'sides of His-RFP without stop codon [BBa_K1332002] . Then, we coupled each BBa_K1859004 and [BBa_K1859006] in 3 'sides. In addition,Lacl,RBS and DT conbined to them. When this device was expressed in E.coli, Curcular mRNA including the linker was made. The efficiency that mRNA is circularized changed by sequence of the mRNA. We let mRNA of RFP circularize and produced a long-chain protein, but the functionality was lost. It is a problem to make a long-chain protein with keeping functionality.

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 We attached this part[BBa_1859001] to 5 'sides of
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-Then, we coupled each BBa_K1859004 and BBa_K1859006 in 3 'sides.
+Then, we coupled each BBa_K1859004 and
+<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859006" > [BBa_K1859006]
+ </a>
+in 3 'sides.
 In addition,Lacl,RBS and DT conbined to them.
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 It is a problem to make a long-chain protein with keeping functionality.
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