Difference between revisions of "Part:BBa K1859001:Experience"

Revision as of 03:14, 18 September 2015

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Applications of BBa_K1859001

User Reviews

UNIQ1ef98378b6357d32-partinfo-00000000-QINU UNIQ1ef98378b6357d32-partinfo-00000001-QINU

[iGEM gifu 2015] .

We attached this part[BBa_1859001] to 5 'sides of His-RFP without stop codon [BBa_K1332002] . Then, we coupled each BBa_K1859004 and BBa_K1859006 in 3 'sides. In addition,Lacl,RBS and DT conbined to them. When this device was expressed in E.coli, Curcular mRNA including the linker was made. The efficiency that mRNA is circularized changed by sequence of the mRNA. We let mRNA of RFP circularize and produced a long-chain protein, but the functionality was lost. It is a problem to make a long-chain protein with keeping functionality.

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 <html>
 <p>
-We attached this part to 5 'sides of
+<a href= "http://2015.igem.org/Team:Gifu" > [iGEM gifu 2015]
+ </a> .
+</p>
+<p>
+We attached this part[BBa_1859001] to 5 'sides of
 His-RFP without stop codon
 <a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332002" > [BBa_K1332002]
   </a> .
-In addition, we coupled mRNA circularization device (3'side) in 3 'sides.
+Then, we coupled each BBa_K1859004 and BBa_K1859006 in 3 'sides.
+In addition,Lacl,RBS and DT conbined to them.
 When this device was expressed in E.coli, Curcular mRNA including the linker was made.
 The efficiency that mRNA is circularized changed by sequence of the mRNA.