Difference between revisions of "Part:BBa K1813004"
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<h2>Background of <i>CYP6G1</i></h2>
 
<h2>Background of <i>CYP6G1</i></h2>
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As an enzyme capable of hydroxylating the 4 and 5 carbons of imidacloprid, it is capable of bestowing a significant protective effect to insects harboring this gene. It has an affinity to 6OH imidacloprid, allowing it to gnerate 4,5 OH imidacloprid, which has an ld50 of over 1 microgram vs 30 nanograms for imidacloprid. Used in conjunction with CYP6CM1vQ,  (BBa_K1813003) it can be extremely effective in bestowing imidacloprid resistance to an organism.
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As an enzyme capable of hydroxylating the 4 and 5 carbons of imidacloprid, it is capable of bestowing a significant protective effect to insects harboring this gene. It has an affinity to 6OH imidacloprid, allowing it to gnerate 4,5 OH imidacloprid [1], which has an ld50 of over 1 microgram vs 57 nanograms for imidacloprid [2]. Used in conjunction with CYP6CM1vQ,  (BBa_K1813003) it can be extremely effective in bestowing imidacloprid resistance to an organism.
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<h2> References </h2>
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[1] Joußen, Nicole, et al. "Metabolism of imidacloprid and DDT by P450 CYP6G1 expressed in cell cultures of Nicotiana tabacum suggests detoxification of these insecticides in Cyp6g1‐overexpressing strains of Drosophila melanogaster, leading to resistance." Pest management science 64.1 (2008): 65-73. <br>
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[2]Suchail, S., Guez, D., & Belzunces, L. P. (2001). Discrepancy between acute and chronic toxicity induced by imidacloprid and its metabolites in Apis mellifera. Environmental toxicology and chemistry, 20(11), 2482-2486.

Revision as of 02:58, 18 September 2015

CYP6G1

CYP6G1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 934
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 943


Background of CYP6G1

As an enzyme capable of hydroxylating the 4 and 5 carbons of imidacloprid, it is capable of bestowing a significant protective effect to insects harboring this gene. It has an affinity to 6OH imidacloprid, allowing it to gnerate 4,5 OH imidacloprid [1], which has an ld50 of over 1 microgram vs 57 nanograms for imidacloprid [2]. Used in conjunction with CYP6CM1vQ, (BBa_K1813003) it can be extremely effective in bestowing imidacloprid resistance to an organism.


References

[1] Joußen, Nicole, et al. "Metabolism of imidacloprid and DDT by P450 CYP6G1 expressed in cell cultures of Nicotiana tabacum suggests detoxification of these insecticides in Cyp6g1‐overexpressing strains of Drosophila melanogaster, leading to resistance." Pest management science 64.1 (2008): 65-73.

[2]Suchail, S., Guez, D., & Belzunces, L. P. (2001). Discrepancy between acute and chronic toxicity induced by imidacloprid and its metabolites in Apis mellifera. Environmental toxicology and chemistry, 20(11), 2482-2486.