Difference between revisions of "Part:BBa K1653001"
 
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''ispDF'' is an operon which made of ''ispD'' and ''ispF'' in ''Escherichia coli''. It was cloned by PCR.
 
''ispDF'' is an operon which made of ''ispD'' and ''ispF'' in ''Escherichia coli''. It was cloned by PCR.
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ispD catalyzes the third step in the methylerythritol phosphate pathway, the CTP-dependent conversion of 2-C-methyl-D-erythritol-4-phosphate to 4-(cytidine 5'-diphospho)-2-C-methyl-erythritol.ispF catalyzes the Mn2+- or Mg2+-dependent conversion of 2-phospho-4-(cytidine 5'diphospho)-2-C-methyl-D-erythritol into 2-C-methyl-D-erythritol-2,4-cyclodiphosphate.
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ispD catalyzes the third step in the methylerythritol phosphate pathway, the CTP-dependent conversion of 2-C-methyl-D-erythritol-4-phosphate to 4-(cytidine 5'-diphospho)-2-C-methyl-erythritol. The ''ispF'' catalyzes the Mn2+- or Mg2+-dependent conversion of 2-phospho-4-(cytidine 5'diphospho)-2-C-methyl-D-erythritol into 2-C-methyl-D-erythritol-2,4-cyclodiphosphate.
  
  

Latest revision as of 02:16, 18 September 2015

ispDF

ispDF is an operon which made of ispD and ispF in Escherichia coli. It was cloned by PCR. ispD catalyzes the third step in the methylerythritol phosphate pathway, the CTP-dependent conversion of 2-C-methyl-D-erythritol-4-phosphate to 4-(cytidine 5'-diphospho)-2-C-methyl-erythritol. The ispF catalyzes the Mn2+- or Mg2+-dependent conversion of 2-phospho-4-(cytidine 5'diphospho)-2-C-methyl-D-erythritol into 2-C-methyl-D-erythritol-2,4-cyclodiphosphate.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1064
    Illegal BamHI site found at 392
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]