Difference between revisions of "Part:BBa K1795000"

 
 
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<partinfo>BBa_K1795000 short</partinfo>
 
<partinfo>BBa_K1795000 short</partinfo>
  
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This sgRNA sequence was designed to target the Promoter Bba_R0010 with the N20 sequence  atgttgtgtggaattgtgag. The part is under the control of the promoter Bba_J23100. If this gRNA is expressed in a cell with a form of the dCas9 protein, the protein will be targeted to the N20 sequence and repress gene expression. Expression of this part is repressed in the presence of LacI and CAP protein.
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This sequence codes for dCas9 and is codon optimized for E. Coli. It will need a Promoter, RBS, and double terminator in order to function.  Thanks to team iGEM13_SJTU-BioX-Shanghai  for original, non-codon optimized sequence.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:32, 18 September 2015

E. Coli Codon Optimized dCAS9, solo

This sequence codes for dCas9 and is codon optimized for E. Coli. It will need a Promoter, RBS, and double terminator in order to function. Thanks to team iGEM13_SJTU-BioX-Shanghai for original, non-codon optimized sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 790
    Illegal PstI site found at 2416
    Illegal PstI site found at 3658
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 790
    Illegal PstI site found at 2416
    Illegal PstI site found at 3658
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 990
    Illegal BglII site found at 1046
    Illegal BglII site found at 1188
    Illegal BglII site found at 1736
    Illegal BglII site found at 3576
    Illegal BglII site found at 3815
    Illegal BamHI site found at 3378
    Illegal BamHI site found at 3739
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 790
    Illegal PstI site found at 2416
    Illegal PstI site found at 3658
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 790
    Illegal PstI site found at 2416
    Illegal PstI site found at 3658
    Illegal NgoMIV site found at 3960
    Illegal AgeI site found at 3304
  • 1000
    COMPATIBLE WITH RFC[1000]