Difference between revisions of "Part:BBa K1795002:Design"

 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K1795001 short</partinfo>
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<partinfo>BBa_K1795002 short</partinfo>
  
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<partinfo>BBa_K1795001 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1795002 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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We made sure to not target the sequence that is promoting the gRNA
 
  
  
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===Source===
 
===Source===
  
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The basic template for the sgRNA sequence was taken from the supplementary information of Qi LS, Larson MH, Gilbert LA, et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013;152(5):1173-1183. doi:10.1016/j.cell.2013.02.022.
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This sequence codes for dCas9 and is codon optimized for E. Coli. It will need a Promoter, RBS, and double terminator in order to function. Thanks to team iGEM13_SJTU-BioX-Shanghai  for original, non-codon optimized sequence.  
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===References===
 
===References===

Revision as of 00:49, 18 September 2015

R0010 gRNA under R0040


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

This sequence codes for dCas9 and is codon optimized for E. Coli. It will need a Promoter, RBS, and double terminator in order to function. Thanks to team iGEM13_SJTU-BioX-Shanghai for original, non-codon optimized sequence.


References