Difference between revisions of "Part:BBa E0022"

Revision as of 23:59, 17 September 2015

enhanced cyan fluorescent protein derived from A. victoria GFP

Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.

Usage and Biology

This part can be used to test promoter strength or the proper functioning of a regulator device. The LVA tail is particularly useful when testing a regulatory device, for example to assess the efficiency of repressor.

In this experiment, the E0022 part was associated with a cI regulated promoter (part R0051), resulting in K1707028.

Figure 1: Fluorescence microscopy of E. coli DH5alpha bearing K170728

Differential interference contrast

Excitation with led 437 nm/17nm (CFP), 100 ms of exposure

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 ===Usage and Biology===
-This part can be used to test promoter strength or the proper functioning of a regulator device.
+This part can be used to test promoter strength or the proper functioning of a regulator device.  The LVA tail is particularly useful when testing a regulatory device, for example to assess the efficiency of repressor.
+In this experiment, the E0022 part was associated with a cI regulated promoter (part R0051), resulting in K1707028.
+'''Figure 1: Fluorescence microscopy of ''E. coli'' DH5alpha bearing K170728'''
+[[File:Paris_Saclay_ K1707028_w1POL.png|300px]]
+Differential interference contrast
+[[File:Paris_Saclay_K1707028_w2Lumencor CFP 434-17.png|300px]]
+Excitation with led 437 nm/17nm (CFP), 100 ms of exposure
 <span class='h3bb'>Sequence and Features</span>