Difference between revisions of "Part:BBa K1692002"
Daniel.kunin (Talk | contribs) |
Daniel.kunin (Talk | contribs) |
||
| Line 14: | Line 14: | ||
[[File:SB2015_styrene_absorbance.png|thumbnail|center|300px|<b>Styrene Absorbance</b>This is where we write]]<br><br> | [[File:SB2015_styrene_absorbance.png|thumbnail|center|300px|<b>Styrene Absorbance</b>This is where we write]]<br><br> | ||
| − | + | [[File:SB2015_tCA_absorbance.png|thumbnail|center|300px|<b>Styrene Absorbance</b>This is where we write]]<br><br> | |
[[File:SB2015_FMN_absorbance.png|thumbnail|right|300px|<b>Flavin Mononucleotide Absorbance</b>This is where we write]]<br><br> | [[File:SB2015_FMN_absorbance.png|thumbnail|right|300px|<b>Flavin Mononucleotide Absorbance</b>This is where we write]]<br><br> | ||
Revision as of 23:48, 17 September 2015
codon optimized FDC with T7 promoter and Flag Tag
Introduction
Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We codon-optimized S. cerevisiae’s FDC gene for expression in E. coli.
Styrene synthesis pathway The enzymes of interest are phenylalanine ammonia lyase (PAL), ferulic acid decarboxylase (FDC), and a flavin prenyltransferase involved in ubiquinone biosynthesis called UbiX. PAL catalyzes the conversion of phenylalanine to trans-cinnamic acid, while FDC catalyzes the conversion of trans-cinnamic acid to styrene. Recently, it has been discovered that a cofactor is required to activate FDC. This cofactor is a product of the reaction between dimethylallyl monophosphate (DMAP) and flavin mononucleotide (FMN), which is catalyzed by the enzyme UbiX.
Background
Reference
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 233
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]