Difference between revisions of "Part:BBa K1766001:Design"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1766001 short</partinfo>
 
<partinfo>BBa_K1766001 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Needs to be used as a fusion protein.
+
This part should be fused to a protein and will not be functional if followed by a BioBrick scar. If 3A or standard assembly is used, the stop codon in the BioBrick scar should be removed by site directed mutagenesis.
 +
For more detail about the making of this part, please visit the [http://2015.igem.org/Team:Stockholm/Results 2015 iGEM Stockholm wiki page] or contact [mailto:l.r.osterberg@gmail.com Linnea Österberg].
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
e. coli.
+
The micF target is found endogenously in ''Escherichia coli''.
  
−
===References===
+
The BBa_K1766001 BioBrick™ was created by iGEM Stockholm, using a gBlocks® gene fragment from Integrated DNA Technologies

Latest revision as of 23:45, 17 September 2015

MicF Target


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part should be fused to a protein and will not be functional if followed by a BioBrick scar. If 3A or standard assembly is used, the stop codon in the BioBrick scar should be removed by site directed mutagenesis. For more detail about the making of this part, please visit the [http://2015.igem.org/Team:Stockholm/Results 2015 iGEM Stockholm wiki page] or contact Linnea Österberg.


Source

The micF target is found endogenously in Escherichia coli.

The BBa_K1766001 BioBrick™ was created by iGEM Stockholm, using a gBlocks® gene fragment from Integrated DNA Technologies