Difference between revisions of "Part:BBa K1707028"

(Usage and Biology)
(Usage and Biology)
Line 8: Line 8:
 
We characterized this part using fluorescence microscopy.
 
We characterized this part using fluorescence microscopy.
  
'''Figure 1: Fluorescence microscopy of ''E. coli'' DH5alpha bearing K170726'''
+
'''Figure 1: Fluorescence microscopy of ''E. coli'' DH5alpha bearing K170728'''
  
 
[[File:Paris_Saclay_ K1707028_w1POL.png|300px]]
 
[[File:Paris_Saclay_ K1707028_w1POL.png|300px]]
 
Differential interference contrast
 
Differential interference contrast
 +
 
[[File:Paris_Saclay_K1707028_w2Lumencor CFP 434-17.png|300px]]
 
[[File:Paris_Saclay_K1707028_w2Lumencor CFP 434-17.png|300px]]
 
excitation with led 437 nm/17nm (CFP), 100 ms of exposure
 
excitation with led 437 nm/17nm (CFP), 100 ms of exposure

Revision as of 23:40, 17 September 2015

CFP-ssRA downstream of a cI regulated promoter

This part was constructed as an intermediate in a construction aiming at verifying that our constructions using cI and cI857 were properly functionning


Usage and Biology

We characterized this part using fluorescence microscopy.

Figure 1: Fluorescence microscopy of E. coli DH5alpha bearing K170728

Paris Saclay K1707028 w1POL.png Differential interference contrast

Paris Saclay K1707028 w2Lumencor CFP 434-17.png excitation with led 437 nm/17nm (CFP), 100 ms of exposure


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]