Difference between revisions of "Part:BBa K1766001"
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The micF target stems from the 5’ untranslated region of the ompF (outer membrane porin F) gene in E. coli. It is the bind site for micF, a non-coding regulatory RNA. MicF prevents translation of ompF when it binds to the micF target [1].   
 
The micF target stems from the 5’ untranslated region of the ompF (outer membrane porin F) gene in E. coli. It is the bind site for micF, a non-coding regulatory RNA. MicF prevents translation of ompF when it binds to the micF target [1].   
  
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BBa_K1766001 consists of the micF target under the control of Anderson promoter BBa_J23101.
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BBa_K1766001 consists of the micF target and Anderson promoter BBa_J23101.
  
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The micF target can be fused to a protein of interest. It is then possible to down-regulate protein expression using MicF [[BBa_K176600]].  
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The micF target is intended to be fused to a protein of interest. It should then be possible to down-regulate protein expression using micF (BBa_K176600).
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The BioBrick has been sequenced and confirmed as the correct construct. Due to time constraints iGEM Stockholm 2015 were unable to caracterize this part.
  
  

Revision as of 23:39, 17 September 2015

MicF Target


The micF target stems from the 5’ untranslated region of the ompF (outer membrane porin F) gene in E. coli. It is the bind site for micF, a non-coding regulatory RNA. MicF prevents translation of ompF when it binds to the micF target [1].

BBa_K1766001 consists of the micF target and Anderson promoter BBa_J23101.

The micF target is intended to be fused to a protein of interest. It should then be possible to down-regulate protein expression using micF (BBa_K176600).

The BioBrick has been sequenced and confirmed as the correct construct. Due to time constraints iGEM Stockholm 2015 were unable to caracterize this part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12