Difference between revisions of "Part:BBa K1758354"
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nickel induceble promoter under the control of a T7 promoter wit a 5´untranslated region and sfGFP for detection.
 
nickel induceble promoter under the control of a T7 promoter wit a 5´untranslated region and sfGFP for detection.
  
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===Usage and Biology===
 
===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1758320 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1758320 SequenceAndFeatures</partinfo>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1758354 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1758354 SequenceAndFeatures</partinfo>

Revision as of 23:35, 17 September 2015

Nickel responsive promoter with T7-promoter and UTR-sfGFP

nickel induceble promoter under the control of a T7 promoter wit a 5´untranslated region and sfGFP for detection.


Usage and Biology

This part is essential for our in vitro characterization of our nickel sensor. The edition of T7 promoter to BBa_K1758353 enables us to characterize tihs sensor in our CFPS.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 198