Difference between revisions of "Part:BBa K1758353"
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1758353 short</partinfo> | <partinfo>BBa_K1758353 short</partinfo> | ||
− | + | nickel repressor under teh control of a constitutive promoter with the induceble promoter a 5´untranslated region infront of the sfGFP which is used for detection. | |
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | <html> | ||
+ | <p> Our Nickel biosensor consists of parts of the rcn-operon from <i> E. coli </i> which codes for a nickel- and cobalt-efflux system. This system is highly sensitive to nickel. In absence of nickel or cobalt RcnR binds to the operator and inhibits the nickel responsive promoter. With Ni(II)-ions present the repression of the promoter RcnA will be reversed, because the repressor RcnR binds nickel-ions and cannot attach to the DNA. For our biosensor we construct the part (<a href="https://parts.igem.org/Part:BBa_K1758353" target="_blank"> BBa_K1758353 </a>by using the basic construction showed in <Our biosensors >. For this part we used the repressor RcnR under control of a constitutive promoter (<a href="https://parts.igem.org/Part:BBa_K1758350" target="_blank"> BBa_K1758350 </a>) and the nickel specific promoter RcnA with a 5’UTR in front of sfGFP (<a href="https://parts.igem.org/Part:BBa_K1758352" target="_blank"> BBa_K1758352 </a>) as reporter protein. </p></html> | ||
− | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1758353 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1758353 SequenceAndFeatures</partinfo> |
Revision as of 23:31, 17 September 2015
Nickel repressor under control of constitutive promoter and strong RBS and Nickel responsive promote
nickel repressor under teh control of a constitutive promoter with the induceble promoter a 5´untranslated region infront of the sfGFP which is used for detection.
Usage and Biology
Our Nickel biosensor consists of parts of the rcn-operon from E. coli which codes for a nickel- and cobalt-efflux system. This system is highly sensitive to nickel. In absence of nickel or cobalt RcnR binds to the operator and inhibits the nickel responsive promoter. With Ni(II)-ions present the repression of the promoter RcnA will be reversed, because the repressor RcnR binds nickel-ions and cannot attach to the DNA. For our biosensor we construct the part ( BBa_K1758353 by using the basic construction showed in
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 509