Difference between revisions of "Part:BBa K1766000"

Latest revision as of 23:28, 17 September 2015

MicF non-coding regulatory RNA

MicF is a non-coding RNA which regulates expression of the ompF (outer membrane porin F) gene in E. coli. MicF binds to the 5’-UTR of ompF mRNA, to prevent translation and promote degradation [1].

BBa_K1766000 is intended to be used together with BBa_K1766001 (MicF Target) to regulate protein expression.

The BioBrick has been sequenced and confirmed as the correct construct. Due to time constraints iGEM Stockholm 2015 were unable to caracterize this part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

[1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12

Revision as of 23:22, 17 September 2015 (view source) Sarahwideman (Talk \| contribs) ← Older edit		Latest revision as of 23:28, 17 September 2015 (view source) Sarahwideman (Talk \| contribs)
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	BBa_K1766000 is intended to be used together with BBa_K1766001 (MicF Target) to regulate protein expression.		BBa_K1766000 is intended to be used together with BBa_K1766001 (MicF Target) to regulate protein expression.

−	Due to time constraints iGEM Stockholm 2015 were unable to caracterize this part.	+	The BioBrick has been sequenced and confirmed as the correct construct. Due to time constraints iGEM Stockholm 2015 were unable to caracterize this part.