Difference between revisions of "Part:BBa K1707026"
(→Usage and Biology) |
|||
| Line 6: | Line 6: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | We | + | We characterized this part using fluorescence microscopy. |
| + | Figure 1: Fluorescence microscopy of ''E. coli'' DH5alpha bearing K170726 | ||
| − | [[File:Paris_Saclay_K1707026_w1POL.png|400px | + | [[File:Paris_Saclay_K1707026_w1POL.png|400px]] |
Differential interference contrast | Differential interference contrast | ||
| − | [[File:Paris_Saclay_K1707026_w2Lumencor CFP 434-17.png|400px | + | [[File:Paris_Saclay_K1707026_w2Lumencor CFP 434-17.png|400px]] |
excitation with led 437 nm/17nm (CFP), 100 ms of exposure | excitation with led 437 nm/17nm (CFP), 100 ms of exposure | ||
Revision as of 23:25, 17 September 2015
CFP-ssRA under the control of J23101
This part was constructed to have a positive control for the expression of CFP-ssRA (under the control of a strong promoter).
Usage and Biology
We characterized this part using fluorescence microscopy. Figure 1: Fluorescence microscopy of E. coli DH5alpha bearing K170726
Differential interference contrast
excitation with led 437 nm/17nm (CFP), 100 ms of exposure
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]