Difference between revisions of "Part:BBa K950001:Experience"
ShuyanTang (Talk | contribs) (→User Reviews) |
|||
| Line 19: | Line 19: | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
<!-- DON'T DELETE --><partinfo>BBa_K950001 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K950001 EndReviews</partinfo> | ||
| + | |||
| + | {|width='80%' style='border:1px solid gray' | ||
| + | |- | ||
| + | |width='10%'| | ||
| + | <partinfo>BBa_K950001 AddReview 3</partinfo> | ||
| + | <I>HUST-China</I> | ||
| + | |width='60%' valign='top'| | ||
| + | |||
| + | |||
| + | [[File: HUST-China_modeling_3.png|thumb|center|400px|Figure 1: The whole circuit]] | ||
| + | |||
| + | <h3>'''Modeling'''</h3> | ||
| + | <h4>Rate of basic transcription for Panb1:</h4> | ||
| + | Since promoter Anb1 has basic transcription rate, Rox1, Si-tag and Mcfp-3 would be expressed even in darkness. Hence, to figure out how it takes effect on our model, we run the simulation with different rate of Panb1 basic transcription (0.1*Vmax to 0.2*Vmax with step of 0.005*Vmax). | ||
| + | |||
| + | [[File: HUST-China_modeling_quick.gif|thumb|center|500px|]] | ||
| + | |||
| + | <h4>Results:</h4> | ||
| + | Our DDEs model is sensitive to the amount of basic transcription of Panb1. If the rate of pAnb1 basic transcription increases from 0.100*Vmax to 0.200*Vmax, the amount of Rox1 would become 125 percent. Therefore, with less basic transcription of Panb1, less Rox1 would be expressed and the repressed expression of Si-tag and Mcfp-3 would recover more quickly. We can conclude that our circuit could be improved by reducing the basic transcription of pAnb1. | ||
| + | |||
| + | |||
| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K1592018 SequenceAndFeatures</partinfo> | ||
| + | |||
| + | |||
| + | |}; | ||
| + | <!-- End of the user review template --> | ||
Revision as of 23:23, 17 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K950001
User Reviews
UNIQ2be13e1a84387f02-partinfo-00000000-QINU UNIQ2be13e1a84387f02-partinfo-00000001-QINU
|
•••
HUST-China |
ModelingRate of basic transcription for Panb1:Since promoter Anb1 has basic transcription rate, Rox1, Si-tag and Mcfp-3 would be expressed even in darkness. Hence, to figure out how it takes effect on our model, we run the simulation with different rate of Panb1 basic transcription (0.1*Vmax to 0.2*Vmax with step of 0.005*Vmax). Results:Our DDEs model is sensitive to the amount of basic transcription of Panb1. If the rate of pAnb1 basic transcription increases from 0.100*Vmax to 0.200*Vmax, the amount of Rox1 would become 125 percent. Therefore, with less basic transcription of Panb1, less Rox1 would be expressed and the repressed expression of Si-tag and Mcfp-3 would recover more quickly. We can conclude that our circuit could be improved by reducing the basic transcription of pAnb1.
Assembly Compatibility:
|
