Difference between revisions of "Part:BBa K1766000"

Revision as of 23:22, 17 September 2015

MicF non-coding regulatory RNA

MicF is a non-coding RNA which regulates expression of the ompF (outer membrane porin F) gene in E. coli. MicF binds to the 5’-UTR of ompF mRNA, to prevent translation and promote degradation [1].

BBa_K1766000 is intended to be used together with BBa_K1766001 (MicF Target) to regulate protein expression.

Due to time constraints iGEM Stockholm 2015 were unable to caracterize this part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

[1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12

@@ Line 5: / Line 5: @@
 MicF is a non-coding RNA which regulates expression of the ompF (outer membrane porin F) gene  in E. coli. MicF binds to the 5’-UTR of ompF mRNA, to prevent translation and  promote degradation [1].
-BBa_K1766000 can be used together with [[BBa_K1766001]] (MicF Target) to regulate protein expression.
+BBa_K1766000 is intended to be used together with BBa_K1766001 (MicF Target) to regulate protein expression.
+Due to time constraints iGEM Stockholm 2015 were unable to caracterize this part.