Difference between revisions of "Part:BBa K950002:Experience"

 
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<partinfo>BBa_K950002 AddReview 3</partinfo>
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<I>HUST-China 2015</I>
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[https://parts.igem.org/Part:BBa_K1592015 '''Pgal1+rox1+CYC1_terminator'''] is used to compose the logic reverse control. GAL1 promoter from Saccharomyces cerevisiae. 1 UAS site allows GAL4 binding domain to enhance the expression from this promoter.
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ROX1 in the downstream of Pgal1 are expressed to inhibit the promoter of anb1.
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===Usage and Biology===
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== '''Application of the part''' ==
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[[File: HUST-China_modeling_3.png|thumb|center|400px|Figure 1: The whole circuit]]
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<h3>'''Modeling'''</h3>
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<h4>Rate of basic transcription for Panb1:</h4>
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Since promoter Anb1 has basic transcription rate, Rox1, Si-tag and Mcfp-3 would be expressed even in darkness. Hence, to figure out how it takes effect on our model, we run the simulation with different rate of Panb1 basic transcription (0.1*Vmax to 0.2*Vmax with step of 0.005*Vmax).
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[[File: HUST-China_modeling_quick.gif|thumb|center|500px|]]
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<h4>Results:</h4>
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Our DDEs model is sensitive to the amount of basic transcription of Panb1. If the rate of pAnb1 basic transcription increases from 0.100*Vmax to 0.200*Vmax, the amount of Rox1 would become 125 percent. Therefore, with less basic transcription of Panb1, less Rox1 would be expressed and the repressed expression of Si-tag and Mcfp-3 would recover more quickly. We can conclude that our circuit could be improved by reducing the basic transcription of Panb1.
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Revision as of 23:08, 17 September 2015


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K950002

User Reviews

UNIQf3b8c85d4f63bc6d-partinfo-00000000-QINU UNIQf3b8c85d4f63bc6d-partinfo-00000001-QINU


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HUST-China 2015

Pgal1+rox1+CYC1_terminator is used to compose the logic reverse control. GAL1 promoter from Saccharomyces cerevisiae. 1 UAS site allows GAL4 binding domain to enhance the expression from this promoter. ROX1 in the downstream of Pgal1 are expressed to inhibit the promoter of anb1.



Application of the part

Figure 1: The whole circuit

Modeling

Rate of basic transcription for Panb1:

Since promoter Anb1 has basic transcription rate, Rox1, Si-tag and Mcfp-3 would be expressed even in darkness. Hence, to figure out how it takes effect on our model, we run the simulation with different rate of Panb1 basic transcription (0.1*Vmax to 0.2*Vmax with step of 0.005*Vmax).

HUST-China modeling quick.gif

Results:

Our DDEs model is sensitive to the amount of basic transcription of Panb1. If the rate of pAnb1 basic transcription increases from 0.100*Vmax to 0.200*Vmax, the amount of Rox1 would become 125 percent. Therefore, with less basic transcription of Panb1, less Rox1 would be expressed and the repressed expression of Si-tag and Mcfp-3 would recover more quickly. We can conclude that our circuit could be improved by reducing the basic transcription of Panb1.


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