Difference between revisions of "Part:BBa K1692002"

Revision as of 23:02, 17 September 2015

codon optimized FDC with T7 promoter and Flag Tag

Introduction

Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We codon-optimized S. cerevisiae’s FDC gene for expression in E. coli.

Styrene synthesis pathwayThis is where we write

Styrene synthesis enzymesThis is where we write

Background

SDS PAGE Gel of Enzymes for Styrene SynthesisThis is a SDS PAGE gel with purified PAL, FDC and UbiX protein. We ran a Mark 12 protein ladder to verify that our proteins were the correct molecular weight.

Styrene AbsorbanceThis is where we write

Flavin Mononucleotide AbsorbanceThis is where we write

Reference

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 233
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 12: / Line 12: @@
 <h2>Background</h2>
-[[File:SB2015_styrene_enzymes_SDS_PAGE.png|thumbnail|center|700px|<b>SDS PAGE Gel of Enzymes for Styrene Synthesis</b>This is where we write]]<br><br>
+[[File:SB2015_styrene_enzymes_SDS_PAGE.png|thumbnail|center|700px|<b>SDS PAGE Gel of Enzymes for Styrene Synthesis</b>This is a SDS PAGE gel with purified PAL, FDC and UbiX protein.  We ran a Mark 12 protein ladder to verify that our proteins were the correct molecular weight.]]<br><br>
-[[File:SB2015_styrene_absorbance.png|thumbnail|center|300px|<b>Styrene Absorbance</b>This is where we write]]<br><br [[File:SB2015_FMN_absorbance.png|thumbnail|right|300px|<b>Flavin Mononucleotide Absorbance</b>This is where we write]]<br><br>
+[[File:SB2015_styrene_absorbance.png|thumbnail|center|300px|<b>Styrene Absorbance</b>This is where we write]]<br><br>
+[[File:SB2015_FMN_absorbance.png|thumbnail|right|300px|<b>Flavin Mononucleotide Absorbance</b>This is where we write]]<br><br>
+<h2>Reference</h2>
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