Difference between revisions of "Part:BBa K1692004"
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Phenylalanine ammonia lyase (PAL) catalyzes the conversion of L-phenylalanine to trans-cinnamic acid. Our PAL construct is codon-optimized for expression in E. coli. The original sequence is derived from Anabaena variabilis. We chose the A. variabilis variant of PAL because the literature has characterized it as functioning well, in contrast to University of British Columbia’s 2013 PAL biobrick part (BBa_K1129003) from Streptomyces maritimus, which has much lower activity. | Phenylalanine ammonia lyase (PAL) catalyzes the conversion of L-phenylalanine to trans-cinnamic acid. Our PAL construct is codon-optimized for expression in E. coli. The original sequence is derived from Anabaena variabilis. We chose the A. variabilis variant of PAL because the literature has characterized it as functioning well, in contrast to University of British Columbia’s 2013 PAL biobrick part (BBa_K1129003) from Streptomyces maritimus, which has much lower activity. | ||
− | [[File:SB2015_styrene_pathway.png|thumbnail|center| | + | [[File:SB2015_styrene_pathway.png|thumbnail|center|500px|<b>Styrene synthesis pathway</b>This is where we write]]<br><br> |
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<h2>Background</h2> | <h2>Background</h2> | ||
− | [[File:SB2015_styrene_enzymes_SDS_PAGE.png|thumbnail|center|700px|<b>SDS PAGE Gel of Enzymes for Styrene Synthesis</b>This is | + | [[File:SB2015_styrene_enzymes_SDS_PAGE.png|thumbnail|center|700px|<b>SDS PAGE Gel of Enzymes for Styrene Synthesis</b>This is a SDS PAGE gel with purified FDC, UbiX and PAL protein. We ran a Mark 12 protein ladder. From this gel we were able to verify that our proteins were the correct molecular weight.]]<br><br> |
Revision as of 22:41, 17 September 2015
codon optimized PAL with T7 promoter and Flag Tag
Introduction
Phenylalanine ammonia lyase (PAL) catalyzes the conversion of L-phenylalanine to trans-cinnamic acid. Our PAL construct is codon-optimized for expression in E. coli. The original sequence is derived from Anabaena variabilis. We chose the A. variabilis variant of PAL because the literature has characterized it as functioning well, in contrast to University of British Columbia’s 2013 PAL biobrick part (BBa_K1129003) from Streptomyces maritimus, which has much lower activity.
Background
Reference
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1315
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1533
- 1000COMPATIBLE WITH RFC[1000]