Difference between revisions of "Part:BBa K1615000"

Revision as of 22:34, 17 September 2015

Morphine-6-dehydrogenase RFC25 The structural gene morphine-6-dehydrogenase (morA) was first isolated from Pseudomonas putida M10 as it is capable of growth with morphine as its sole carbon source¹. Morphine dehydrogenase catalyses the oxidation of both morphine and codeine to produce morphinone and codeinone respectively. During this process NADP⁺ is reduced to NADPH which means that this enzyme is frequently used to detect morphine and codeine².

To test the morphine dehydrogenase activity it can be coupled with morphine and NADP⁺ to produce morphinone and NADPH. The amount of NADPH produced can be measured at 340nm. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 840
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1615000 short</partinfo>
+The structural gene morphine-6-dehydrogenase (<i>morA</i>) was first isolated from <i>Pseudomonas putida</i> M10 as it is capable of growth with morphine as its sole carbon source<sup>1</sup>. Morphine dehydrogenase catalyses the oxidation of both morphine and codeine to produce morphinone and codeinone respectively. During this process NADP<sup>+</sup> is reduced to NADPH which means that this enzyme is frequently used to detect morphine and codeine<sup>2</sup>.
-Morphine-6-dehydrogenase. NADP dependent oxidoreductase. Oxidises morphine to morphinone.
+<br>
+<br>
+To test the morphine dehydrogenase activity it can be coupled with morphine and NADP<sup>+</sup> to produce morphinone and NADPH. The amount of NADPH produced can be measured at 340nm.
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 ===Usage and Biology===