Difference between revisions of "Part:BBa K1692004"
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<h2>Introduction</h2>
 
<h2>Introduction</h2>
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Phenylalanine ammonia lyase (PAL) catalyzes the conversion of L-phenylalanine to trans-cinnamic acid. Our PAL construct is codon-optimized for expression in E. coli. The original sequence is derived from Anabaena variabilis. We chose the A. variabilis variant of PAL because the literature has characterized it as functioning well, in contrast to University of British Columbia’s 2013 PAL biobrick part (BBa_K1129003) from Streptomyces maritimus, which has much lower activity.
  
 
Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We codon-optimized S. cerevisiae’s FDC gene for expression in E. coli.  
 
Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We codon-optimized S. cerevisiae’s FDC gene for expression in E. coli.  
  
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[[File:SB2015_styrene_pathway.png|thumbnail|right|300px|<b>Styrene synthesis pathway</b>This is where we write]]<br><br>
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[[File:SB2015_styrene_pathway.png|thumbnail|left|400px|<b>Styrene synthesis pathway</b>This is where we write]]<br><br>
  
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[[File:SB2015_styrene_enzymes.png|thumbnail|center|500px|<b>Styrene synthesis enzymes</b>This is where we write]]<br><br>
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[[File:SB2015_styrene_enzymes.png|thumbnail|right|300px|<b>Styrene synthesis enzymes</b>This is where we write]]<br><br>
  
 
<h2>Background</h2>
 
<h2>Background</h2>

Revision as of 22:30, 17 September 2015

codon optimized PAL with T7 promoter and Flag Tag

Introduction

Phenylalanine ammonia lyase (PAL) catalyzes the conversion of L-phenylalanine to trans-cinnamic acid. Our PAL construct is codon-optimized for expression in E. coli. The original sequence is derived from Anabaena variabilis. We chose the A. variabilis variant of PAL because the literature has characterized it as functioning well, in contrast to University of British Columbia’s 2013 PAL biobrick part (BBa_K1129003) from Streptomyces maritimus, which has much lower activity.

Ferulic acid decarboxylase (FDC) catalyzes the conversion of trans-cinnamic acid to styrene. We codon-optimized S. cerevisiae’s FDC gene for expression in E. coli.

Styrene synthesis pathwayThis is where we write


Styrene synthesis enzymesThis is where we write


Background

SDS PAGE Gel of Enzymes for Styrene SynthesisThis is where we write



TitleThis is where we write


PAL FunctionalityThis is where we write


Reference

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1315
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1533
  • 1000
    COMPATIBLE WITH RFC[1000]