Difference between revisions of "Part:BBa K1723001"
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<b>PAM rich URS J23117 is an improvement of the J23117 (BBa_J23117) weak promoter</b> [1]. This promoter is the promoter J23117 flanked with a PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence (URS) to enable the use of protein dCas9-&#969; (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: the activator sgRNA Z0 (BBa_K1723002), the inhibitor sgRNA Z35 (BBa_K1723003), or the other inhibitor sgRNA Z4 (BBa_K1723004). dCas9 can only bind if its target sequence is preceded PAM sequence. Discover the applications of this part in the tab 'experience'.  
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<b>PAM rich URS J23117 is an improvement of the J23117 (BBa_J23117) weak promoter</b> [1]. This promoter is the promoter J23117 flanked with a PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence (URS) to enable the use of protein dCas9-&#969; (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: the activator sgRNA Z4 (BBa_K1723003), the inhibitor sgRNA Z0 (BBa_K1723002), or the other inhibitor sgRNA Z35 (BBa_K1723004). dCas9 can only bind if the DNA target sequence is preceded by a PAM sequence.  
  
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Discover more parts that can work with this one:
  
 
http://2015.igem.org/Team:EPF_Lausanne/Part_Collection
 
http://2015.igem.org/Team:EPF_Lausanne/Part_Collection

Revision as of 21:49, 17 September 2015

PAM rich URS j23117 promoter


PAM rich URS J23117 is an improvement of the J23117 (BBa_J23117) weak promoter [1]. This promoter is the promoter J23117 flanked with a PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence (URS) to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: the activator sgRNA Z4 (BBa_K1723003), the inhibitor sgRNA Z0 (BBa_K1723002), or the other inhibitor sgRNA Z35 (BBa_K1723004). dCas9 can only bind if the DNA target sequence is preceded by a PAM sequence.

Discover more parts that can work with this one:

http://2015.igem.org/Team:EPF_Lausanne/Part_Collection

EPFL_Lausanne_promoter.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 56
    Illegal NheI site found at 79
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 43
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic acids research, 41(15), 7429-7437.