Difference between revisions of "Part:BBa K207001:Experience"

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[[File:Yeast two-hybrid light control.png|600px|thumb|center| Figure 1:light control system]]
 
<h2>'''Modeling'''</h2>
 
<h2>'''Modeling'''</h2>
 
Before the circuit was determined, there were two kinds of light control system for choice: the CRY2-CIB1 system and the PhyA-FHL system. To find out the system that fits our circuit better, we simulated both of them with the DDEs model.
 
Before the circuit was determined, there were two kinds of light control system for choice: the CRY2-CIB1 system and the PhyA-FHL system. To find out the system that fits our circuit better, we simulated both of them with the DDEs model.
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[[File:HUST-China_2015_modeling_1_new.png|600px|thumb|center|Figure 1.1: Simulation of PhyA-FHL system]]
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[[File:HUST-China_2015_modeling_1_new.png|600px|thumb|center|Figure 2.1: Simulation of PhyA-FHL system]]
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[[File:HUST-China_2015_modeling_2.png|600px|thumb|center| Figure 1.2: Simulation of CRY2-CIB1 system]]
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[[File:HUST-China_2015_modeling_2.png|600px|thumb|center| Figure 2.2: Simulation of CRY2-CIB1 system]]
  
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'''The figure 1.1 and 1.2 shows the following facts:'''
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'''The figure 2.1 and 2.2 shows the following facts:'''
 
<p>    1. The values of (active)PhyA, (active)FHL, (active)CRY2, (active)CIB1 are relatively low and remains at a certain level (approximately 0~7nM). </p>
 
<p>    1. The values of (active)PhyA, (active)FHL, (active)CRY2, (active)CIB1 are relatively low and remains at a certain level (approximately 0~7nM). </p>
 
<p>    2. The peak of CRY2-CIB1 system appears earlier than the one of PhyA-FHL system. </p>
 
<p>    2. The peak of CRY2-CIB1 system appears earlier than the one of PhyA-FHL system. </p>

Revision as of 21:27, 17 September 2015


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Applications of BBa_K207001

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Figure 1:light control system

Modeling

Before the circuit was determined, there were two kinds of light control system for choice: the CRY2-CIB1 system and the PhyA-FHL system. To find out the system that fits our circuit better, we simulated both of them with the DDEs model.

Figure 2.1: Simulation of PhyA-FHL system
Figure 2.2: Simulation of CRY2-CIB1 system

The figure 2.1 and 2.2 shows the following facts:

1. The values of (active)PhyA, (active)FHL, (active)CRY2, (active)CIB1 are relatively low and remains at a certain level (approximately 0~7nM).

2. The peak of CRY2-CIB1 system appears earlier than the one of PhyA-FHL system.

3. The value of Rox1 in CRY2-CIB1 system decreases faster than the one in PhyA-FHL system.


We can safely derive the following conclusions from the figures above.

1. The photoactive subjects are of low concentration but they remain at a certain level.

2. Compared to the PhyA-FHL system, the CRY2-CIB1 system is more sensitive to light exposure (The peak of CRY2-CIB1 system appears earlier than the one of PhyA-FHL system) and the PhyA-FHL system has a time-lag for photoactivation.

3. The rate of Rox1 degradation in CRY2-CIB1 system is higher than the one in PhyA-FHL system, which means the darkness induction could shut down quickly so that the downstream systems could be activated.

Hence, we considered CRY2-CIB1 system more advantageous and applied it to our project.

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