Difference between revisions of "Part:BBa K1587008"

Revision as of 21:10, 17 September 2015

Red light biosensor

Strong promoter BBa_J23119.Strong RBS BBa_B0030 Chimeric Red light receptor cph8 BBa_I15010.

Figure 1: Chimeric Red light receptor cph8 genetic construct

Figure 2: Chimeric Red light receptor cph8 (cph1-envZ) is inactivated by phycobilin PCB photosynthetic light-harvesting complexes preventing the phosphorylation of ompR

Phosphorylation of ompR by cph8 inhibits the expression of the genes regulated by promoter pompR. Since ompR and pompR are naturaly found in E.coli, this biobrick enables a circadian expression of genes regulated by pompR in E. coli straine deleted for envZ.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 414
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 <center>[[File:TLSE Circ3.jpg]]</center>
 <center>'''Figure 1: Chimeric Red light receptor cph8  genetic construct'''</center>
 <center>[[File:TLSE CircSchem.jpg]]</center>
 <center>'''Figure 2: Chimeric Red light receptor cph8 (cph1-envZ) is inactivated by phycobilin PCB photosynthetic light-harvesting complexes preventing the phosphorylation of ompR'''</center>
+Phosphorylation of ompR by cph8 inhibits the expression of the genes regulated by promoter pompR. Since ompR and pompR are naturaly found in ''E.coli'', this biobrick enables a circadian expression of genes regulated by pompR in E. coli straine deleted for envZ.
 <!-- Add more about the biology of this part here