Difference between revisions of "Part:BBa K1791001:Design"
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===Source=== | ===Source=== | ||
| − | This is an amalgamation of several biobricks an MS2 coat protein and | + | This is an amalgamation of several biobricks an MS2 coat protein, a MS2 RNA apatamer and a theophylline ribozyme (BBa_K1791000) |
===References=== | ===References=== | ||
Latest revision as of 21:08, 17 September 2015
Standard RBS expressing MS2 Coat protein ribozyme affinity purification (RAP)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 603
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 95
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We had to consider MS2 expression levels relative to the theophylline ribozyme transcript and assessed how this affects total RNA yield. In order to test this we developed a second part with depressed MS2 expression (BBa_K1791002)
Source
This is an amalgamation of several biobricks an MS2 coat protein, a MS2 RNA apatamer and a theophylline ribozyme (BBa_K1791000)