Difference between revisions of "Part:BBa K1679016:Design"
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===References=== | ===References=== | ||
Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265. | Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265. | ||
Revision as of 21:03, 17 September 2015
Promoter+RNA thermometer (ROSE)+mRFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 700
Illegal AgeI site found at 812 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 42°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above 42°C, the secondary structure of the RBS after transform would be unfolded and produce RFP.
Source
BBa_J23101
BBa_K115001
BBa_E1010
References
Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265.