Difference between revisions of "Part:BBa K1486003"
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Flexible linker used to fuse two proteins. | Flexible linker used to fuse two proteins. | ||
| − | <!-- Add more about the biology of this part here | + | |
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| − | Group: iGEM15_Fudan | + | Group: iGEM15_Fudan/n |
| − | Author: Xuhang Li | + | Author: Xuhang Li/n |
Summary:We developed a new part(BBa_K1777005) based on this parts. The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR. | Summary:We developed a new part(BBa_K1777005) based on this parts. The codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Besides ,we lengthen this linker to provide better separation of two domains. At last we avoid tandem repeat sequence(like GGGSPKKKRKVGGGS), which will facilitate fusing this linker to your domains via overlap PCR. | ||
| + | ===Usage and Biology=== | ||
| + | |||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 19:43, 17 September 2015
Flexible linker 2x (GGGS)
Flexible linker used to fuse two proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]