Difference between revisions of "Part:BBa K1766007"
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This part is a combination of OmpR regulated RhlI (produces a quorum sensing molecule, BHL), micF regulated LuxI (produces a quorum sensing molecule, OHHL) and micF RNA. The part was designed to achieve a highly sensitive on-off switch mechanism in the OmpR dependent regulatory system. | This part is a combination of OmpR regulated RhlI (produces a quorum sensing molecule, BHL), micF regulated LuxI (produces a quorum sensing molecule, OHHL) and micF RNA. The part was designed to achieve a highly sensitive on-off switch mechanism in the OmpR dependent regulatory system. | ||
| − | The part is designed by iGEM Stockholm team 2015 but could not be created in practice due to lack of time. The function of this part is aimed on the kinase and the phosphatase activity of the EnvZ-Affibody receptor. The purpose is to produce BHL in high osmolarity condition when phosphorylated OmpR expresses RhlI. In low osmolarity condition, it is supposed to produce OHHL as the micF binds to its target site, leading to LuxI expression. | + | The part is designed by iGEM Stockholm team 2015 but could not be created in practice due to lack of time. The function of this part is aimed to depend on the kinase and the phosphatase activity of the EnvZ-Affibody receptor. The purpose is to produce BHL in high osmolarity condition when phosphorylated OmpR expresses RhlI. In low osmolarity condition, it is supposed to produce OHHL as the micF binds to its target site, leading to LuxI expression. |
[[File:K1766007.png|700px|thumb|center|OmpR Regulated RhlI/LuxI]] | [[File:K1766007.png|700px|thumb|center|OmpR Regulated RhlI/LuxI]] | ||
Revision as of 19:21, 17 September 2015
OmpR Regulated RhlI/LuxI
This part is a combination of OmpR regulated RhlI (produces a quorum sensing molecule, BHL), micF regulated LuxI (produces a quorum sensing molecule, OHHL) and micF RNA. The part was designed to achieve a highly sensitive on-off switch mechanism in the OmpR dependent regulatory system.
The part is designed by iGEM Stockholm team 2015 but could not be created in practice due to lack of time. The function of this part is aimed to depend on the kinase and the phosphatase activity of the EnvZ-Affibody receptor. The purpose is to produce BHL in high osmolarity condition when phosphorylated OmpR expresses RhlI. In low osmolarity condition, it is supposed to produce OHHL as the micF binds to its target site, leading to LuxI expression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1266
Illegal NheI site found at 1289 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1150
Illegal BglII site found at 2098 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]