Difference between revisions of "Part:BBa K907004:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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<partinfo>BBa_K907004 AddReview 5</partinfo>
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<I>BIT-China 2015</I>
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<h2>Fluorescence verification of device K907004 (BIT-China)</h2>
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To verify the expression of the recombinase Bxb1 gf35, we used the part BBa_K907004 as a reported device. In this device, we chose <i>pTetR</i> as the promoter which could be induced by tetracycline. We transformed it into <i>E.coli</i> BMTOP10, and this is our fluorescence result of K907004 verification.
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<img src="https://static.igem.org/mediawiki/2015/1/13/BIT_China_Regulation_System_pic31.png" style="width:855px;">
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According to the picture, we can obviously find that the fluorescence verification system K907004 is working. Meanwhile, we also cam prove the function of Bxb1 gf35.

Revision as of 18:57, 17 September 2015

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Please enter how you used this part and how it worked out.

Applications of BBa_K907004

User Reviews

UNIQ6d6049de216a42e3-partinfo-00000000-QINU UNIQ6d6049de216a42e3-partinfo-00000001-QINU


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•••••

BIT-China 2015 |width='60%' valign='top'|

Fluorescence verification of device K907004 (BIT-China)

To verify the expression of the recombinase Bxb1 gf35, we used the part BBa_K907004 as a reported device. In this device, we chose pTetR as the promoter which could be induced by tetracycline. We transformed it into E.coli BMTOP10, and this is our fluorescence result of K907004 verification.

According to the picture, we can obviously find that the fluorescence verification system K907004 is working. Meanwhile, we also cam prove the function of Bxb1 gf35.