Difference between revisions of "Part:BBa K1745001:Design"
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The KaiABC circadian oscillator is endogenous to the cynanobacterial species Synechococcus elongatus. | The KaiABC circadian oscillator is endogenous to the cynanobacterial species Synechococcus elongatus. | ||
+ | ===References=== | ||
Chen, A. H., Lubkowicz, D., Yeong, V., Chang, R. L., & Silver, P. a. (2015a). Transplantability of a circadian clock to a noncircadian organism. Science Advance, 1(5), 1–6. http://doi.org/10.1126/sciadv.1500358 | Chen, A. H., Lubkowicz, D., Yeong, V., Chang, R. L., & Silver, P. a. (2015a). Transplantability of a circadian clock to a noncircadian organism. Science Advance, 1(5), 1–6. http://doi.org/10.1126/sciadv.1500358 |
Revision as of 18:16, 17 September 2015
KaiABC Oscillator
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3014
Illegal NheI site found at 3037 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 410
Illegal XhoI site found at 477 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2945
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 540
Design Notes
In vitro investigations of this modular system revealed that the robustness of oscillations was sensitive to stoichiometric ratios of KaiA to KaiC (Nakajima, Science 2015). To experiment with these stoichiometric ratios, KaiB and KaiC expression are driven under a constitutive promoter (Part J23100) while KaiA expression is driven under a L-rhamnose inducible promoter (Part K914003).
Source
The KaiABC circadian oscillator is endogenous to the cynanobacterial species Synechococcus elongatus.
References
Chen, A. H., Lubkowicz, D., Yeong, V., Chang, R. L., & Silver, P. a. (2015a). Transplantability of a circadian clock to a noncircadian organism. Science Advance, 1(5), 1–6. http://doi.org/10.1126/sciadv.1500358
Nakajima, M., Imai, K., Ito, H., Nishiwaki, T., Murayama, Y., Iwasaki, H., … Kondo, T. (2005). Reconstitution of circadian oscillation of cyanobacterial KaiC phosphorylation in vitro. Science (New York, N.Y.), 308(5720), 414–415. http://doi.org/10.1126/science.1108451
Nakajima, M., Ito, H., & Kondo, T. (2010). In vitro regulation of circadian phosphorylation rhythm of cyanobacterial clock protein KaiC by KaiA and KaiB. FEBS Letters, 584(5), 898–902. http://doi.org/10.1016/j.febslet.2010.01.016
Pattanayak, G., & Rust, M. J. (2014). The cyanobacterial clock and metabolism. Current Opinion in Microbiology, 18(1), 90–95. http://doi.org/10.1016/j.mib.2014.02.010
Rust, M. J., Markson, J. S., Lane, W. S., Fisher, D. S., & O’Shea, E. K. (2007). Ordered phosphorylation governs oscillation of a three-protein circadian clock. Science (New York, N.Y.), 318(5851), 809–812. http://doi.org/10.1126/science.1148596
Taniguchi, Y., Takai, N., Katayama, M., Kondo, T., & Oyama, T. (2010). Three major output pathways from the KaiABC-based oscillator cooperate to generate robust circadian kaiBC expression in cyanobacteria. Proceedings of the National Academy of Sciences of the United States of America, 107(7), 3263–3268. http://doi.org/10.1073/pnas.0909924107