Difference between revisions of "Part:BBa K1797004"

 
 
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<partinfo>BBa_K1797004 short</partinfo>
 
<partinfo>BBa_K1797004 short</partinfo>
  
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CcdB protein is a topoisomerase poision which affects DNA gyrase and inhibits the segregation of the chromosomal DNA.
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This part is an improved part based on BBa_K1797000. Except for the GFP reporter system, we introduced cI repressor and corresponding BFP. In the absence of blue light, GFP but not BFP will be expressed. With blue light, BFP but not GFP will be expressed.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:45, 17 September 2015

Blue Light Sensing System With GFP And BFP Reporter

This part is an improved part based on BBa_K1797000. Except for the GFP reporter system, we introduced cI repressor and corresponding BFP. In the absence of blue light, GFP but not BFP will be expressed. With blue light, BFP but not GFP will be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1955
    Illegal NheI site found at 1978
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2553
    Illegal NgoMIV site found at 2625
    Illegal NgoMIV site found at 2715
    Illegal NgoMIV site found at 2733
    Illegal NgoMIV site found at 3245
    Illegal NgoMIV site found at 3538
    Illegal NgoMIV site found at 3632
    Illegal AgeI site found at 2267
    Illegal AgeI site found at 3413
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3302
    Illegal BsaI.rc site found at 2166