Difference between revisions of "Part:BBa K1602017"
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− | <h1> | + | <h1><small>D</small>-xylonic acid producing operon</h1> |
− | + | <b><small>D</small>-Xylose</b> is a monosaccharide belonging to the aldopentose family. It was recently shown that the <small>D</small>-xylose dehydrogenase <i>xylB</i> from <i>Caulobacter crescentus</i> can convert <small>D</small>-xylose to <small>D</small>-xylonolactone. This can react spontaneously or through the catalysation of <i>xylC</i> to <small>D</small>-xylonic acid. In <i>E. coli</i> <small>D</small>-xylonic acid can be further metabolized to ethyleneglycol. | |
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− | <img style="width: 565px; height: 110px; margin-left: 15px; margin-right: 15px;" alt="" src="https://static.igem.org/mediawiki/2015/7/7b/ | + | <img style="width: 565px; height: 110px; margin-left: 15px; margin-right: 15px;" alt="" src="https://static.igem.org/mediawiki/2015/7/7b/Conversion_ xylose_xylitol_v1.png"></div> |
<p style="width: 900px; margin-left: 15px; margin-right: 15px;" alt="" text-align="left"> | <p style="width: 900px; margin-left: 15px; margin-right: 15px;" alt="" text-align="left"> | ||
− | <b>Figure 1</b> | + | <b>Figure 1</b> |
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===<h2>Usage</h2>=== | ===<h2>Usage</h2>=== | ||
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− | This part is a composite of | + | This part is a composite of two coding genes with strong RBS (<a href="/Part:BBa_B0034">BBa_B0034</a>). The transcription is controlled by a T7 promotor (<a href="/Part:BBa_I719005">BBa_I719005</a>). |
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− | + | <li class="block-10vi">T7-promotor | |
+ | <a href="/Part:BBa_I719005">(BBa_I719005)</a></li> | ||
+ | |||
+ | <li class="block-10vi">ribosome binding site B0034 | ||
+ | <a href="/Part:BBa_B0034">(BBa_B0034)</a></li> | ||
+ | |||
+ | <li class="block-10vi"><small>D</small>-xylose dehydrogenase xylB | ||
+ | <a href="/Part:BBa_K1602009">(BBa_K1602009)</a></li> | ||
+ | |||
+ | <li class="block-10vi"><small>D</small>-xylonolactone lactonase | ||
+ | xylC | ||
+ | <a href="/Part:BBa_K1602010">(BBa_K1602010)</a></li> | ||
+ | |||
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<td align="center" valign="middle"> | <td align="center" valign="middle"> | ||
− | <img style="width: 332px; height: 200px;" alt="" src="https://static.igem.org/mediawiki/2015/7/7e/ | + | <img style="width: 332px; height: 200px;" alt="" src="https://static.igem.org/mediawiki/2015/7/7e/T7_gre3_ operon_v1.png" align="center"> |
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<div style="float: right;"><img style="width: 400px; height: 120px;" alt="" src=" | <div style="float: right;"><img style="width: 400px; height: 120px;" alt="" src=" | ||
− | https://static.igem.org/mediawiki/2015/a/a1/ | + | https://static.igem.org/mediawiki/2015/a/a1/Itaconic_acid_gene_ operon.png"> </div> |
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− | <b>Figure 2</b> | + | <b>Figure 2</b> |
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</tr> | </tr> | ||
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===<h2>Sequence and Features</h2>=== | ===<h2>Sequence and Features</h2>=== | ||
− | <partinfo> | + | <partinfo>BBa_K1602017 SequenceAndFeatures</partinfo> |
Revision as of 17:26, 17 September 2015
D-xylonic acid producing operon
D-Xylose is a monosaccharide belonging to the aldopentose family. It was recently shown that the D-xylose dehydrogenase xylB from Caulobacter crescentus can convert D-xylose to D-xylonolactone. This can react spontaneously or through the catalysation of xylC to D-xylonic acid. In E. coli D-xylonic acid can be further metabolized to ethyleneglycol.![](https://static.igem.org/mediawiki/2015/7/7b/Conversion_ xylose_xylitol_v1.png)
Figure 1
Usage
This part is a composite of two coding genes with strong RBS (BBa_B0034). The transcription is controlled by a T7 promotor (BBa_I719005).
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Figure 2 |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]