Difference between revisions of "Part:BBa K1616007"
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Holin works with endolysin; they create a toxic system, after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bläsi, 1995) | Holin works with endolysin; they create a toxic system, after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bläsi, 1995) | ||
Revision as of 16:17, 17 September 2015
Holin reversed
Holin works with endolysin; they create a toxic system, after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bläsi, 1995)
This part is the reversed sequence of the holin (BBa_K112805); thus this part can be used with a reversed endolysin (BBa_K1616006), reversed promoter, reversed RBS and reversed terminator.
This part is the reverses sequence of the BBa_K112805 (Holin).
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The illegal sites have been checked
Source
This part is the reverses sequence of the BBa_K112805 (Holin).